E-ISSN 2534-9821
 

Original Research (Original Article) 


Protective Effect of Mangosteen Skin Extract on Albino Mice Skin with Dimethyl-Benz(a)anthracene (DMBA) Induction: Analysis on p53 Protein Level

Dyah Pratiwi Purnaningsih, Khairuddin Djawad, Siswanto Wahab, Nasrum Massi, Gemini Alam, Burhanuddin Bahar.

Abstract
Introduction: Skin cancer is the most common type of malignancy in humans with a dramatic incidence increase in the past decade. One of the genes that may play a role in carcinogenesis is p53 gene. Mangosteen skin extract contains xanthone derivatives, such as α-, β-, and γ-mangostins, which are known to possess anticancer property. Despite its well documented use in other types of carcinoma, its effect in skin tumor carcinogenesis is still unknown.
Method: This experimental study was done in 30 mice randomly assigned into groups receiving different treatments. The positive control group received Dimethyl-Benz(a)anthracene (DMBA), a potent carcinogen, induction while the treatment groups received mangosteen skin extract with 100, 200, and 400 ppm concentrations. The protein p53 level from skin tumor in each mouse was assessed using ELISA.
Result: Protein p53 level was highest in the positive control group while the lowest value was shown by the group with 100 ppm extract application. One-way ANOVA test showed a significant difference across groups (p=0.000). Least significant difference post-hoc analysis showed a significantly lower protein p53 levels of both 100 ppm and 400 ppm mangosteen skin extract compared to the DMBA control group (p=0.000).
Conclusion: This study showed that topical application of mangosteen skin extract provided protective effect to skin tumor in mice, possibly through its antioxidant effects. This finding may expand potential future therapeutic options in treating skin cancers.

Key words: DMBA, mangosteen skin extract, p53, skin cancer


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Dyah Pratiwi Purnaningsih
Articles by Khairuddin Djawad
Articles by Siswanto Wahab
Articles by Nasrum Massi
Articles by Gemini Alam
Articles by Burhanuddin Bahar
on Google
on Google Scholar
Article Statistics
 Viewed: 1902
Downloaded: 225


REFERENCES
1. Brennan AM, Mantzoros CS. Drug Insight: the role of leptin in human physiology and pathophysiology-emerging clinical applications. Nat Clin Pract Endocrinol Metab 2006; 2:318-27. [DOI via Crossref]    [Pubmed]   
2. Venkatasubramanian G, Chittiprol S, Neelakantachar N, Shetty TK, Bangalore N, Gangadhar BN. A Longitudinal Study on the Impact of Antipsychotic Treatment on Serum Leptin in Schizophrenia. Clin Neuropharm 2010; 33:288-92. [DOI via Crossref]    [Pubmed]   
3. Corsonello A, Corica F. Leptin, obesity and platelet responsiveness: another piece in the puzzle. Thromb Haemost 2008; 100:960-1.
4. Crowley VE. Overview of human obesity and central mechanisms regulating energy homeostasis. Ann Clin. Biochem 2008; 45:245-55.
5. Hafner SA, Zierer A, Emeny RT, Thorand B, Herder C, Koenig W, et. al.Social isolation and depressed mood are associated with elevated serum leptin levels in men but not in women. Psychoneuroendocrinology 2011; 36:200-9. [DOI via Crossref]    [Pubmed]   
6. Rutters F, Nieuwenhuizen AG, Verhoef SP, Lemmens SG, Vogels N, Westerterp-Plantenga MS. The relationship between leptin, gonadotropic hormones, and body composition during puberty in a Dutch children cohort. Eur J Endocrinol 2009; 160:973-8. [DOI via Crossref]    [Pubmed]   
7. Velloso LA, Savino W, Mansour E. Leptin action in the thymus. Ann N Y Acad. Sci 2009; 1153:29-34.
8. Harvey, J., Ashford, M.L., 2003. Leptin in the CNS: much more than a satiety signal. Neuropharmacology. 44, 845-854.
9. Paz-Filho, G.J., Babikian, T., Asarnow, R., Esposito, K., Erol, H.K., Wong, M., Licinio, J., Leptin Replacement Improves Cognitive Development. 2008. PLoS ONE. 3,1-7.
10. Atmaca, M., Kuloglu, M., Tezcan, E., Ustundag, B., 2008. Serum leptin and cholesterol values in violent and non-violent suicide attempters. Psychiatry Research.158, 87-91.
11. Atmaca, M., Kuloglu, M., Tezcan, E., Gecici, O., Ustundag, B., 2002a. Serum cholesterol and leptin levels in patients with borderline personality disorder. Neuropsychobiology 45, 167-171.
12. Atmaca, M., Kuloglu, M., Tezcan, E., Semercioz, A., Ustundag, B., Ayar, A., 2002b. Serum leptin levels in the patients with premature ejaculation. Archives of Andrology 48, 345-350.
13. Atmaca, M., Kuloglu, M., Tezcan, E., Ustundag, B., 2002c. Serum cholesterol and leptin levels in patients on lithium treatment. Neuropsychobiology 46, 67-69.
14. Kraus, T., Haack, M., Schuld, A., Hinze-Selch, D., Pollmacher, T., 2001. Low leptin levels but normal body mass indices in patients with depression or schizophrenia. Neuroendocrinology 73, 243-247.
15. Harvey, J., Solovyova, N., Irving, A., 2006. Leptin and its role in hippocampal synaptic plasticity. Prog Lipid Res. 45, 369-378.
16. Wilson, M.E., Fisher, J., Brown, J., 2005. Chronic subcutaneous leptin infusion diminishes the responsiveness of the hypothalamic-pitu-itary-adrenal (HPA) axis in female rhesus monkeys. Physiol. Behav. 84, 449—458.
17. Leibowitz, S.F., Alexander, J.T., 1998. Hypothalamic serotonin in control of eating behavior, meal size and body weight. Biol Psychiatry. 44, 851- 864.
18. Brennan, A.M., Fargnoli, J.L., Williams, C.J., Li, T., Willett, W., Kawachi, I., Qi, L., Hu, F.B., Mantzoros, C.S., 2009. Phobic anxiety is associated with higher serum concentrations of adipokines and cytokines in women with diabetes. Diabetes Care 32, 926—931.
19. Narita, K., Murata, T., Hamada, T., Kosaka, H., Sudo, S., Mizukami, K., Yoshida, H., Wada, Y., 2008. Associations between trait anxiety, insulin resistance, and atherosclerosis in the elderly: a pilot cross-sectional study. Psychoneuroendocrinology 33, 305—312.
20. Liao, S.C., Lee, M.B., Lee, Y.J., Huang, T.S., 2004. Hyperleptinemia in subjects with persistent partial posttraumatic stress disorder after a major earthquake. Psychosom. Med. 66, 23—28.
21. Otsuka, R., Yatsuya, H., Tamakoshi, K., Matsushita, K., Wada, K., Toyoshima, H., 2006. Perceived psychological stress and serum leptin concentrations in Japanese men. Obesity 14 (Silver. Spring), 1832—1838.
22. Antonijevic, I.A., Murck, H., Frieboes, R.M., Horn, R., Brabant, G., Steiger, A., 1998. Elevated nocturnal profiles of serum leptin in patients with depression. J. Psychiatr. Res. 32, 403-410.
23. Deuschle, M., Blum, W.F., Englaro, P., Schweiger, U., Weber, B., Pflaum, C.D., Heuser, I., 1996. Plasma leptin in depressed patients and healthy controls. Horm. Metab. Res. 28, 714-717.
24. Esel, E., Ozsoya, S.,Tutus, A., Sofuoglu, S., Kartalci, S.,Bayram, F., Kokbudaka, Z., Kula, M., 2005. Effects of antidepressant treatment and of gender on serum leptin levels in patients with major depression. Progress in Neuro-Psychopharmacology & Biological Psychiatry. 29, 565-570.
25. Rubin, R.T., Rhodes, M.E., Czambel, R.K., 2002. Sexual diergism of baseline plasma leptin and leptin suppression by arginine vasopressin in major depressives and matched controls. Psychiatry Res. 113, 255-268.
26. Atmaca, M., Kuloglu, M., Tezcan, E., Ustundag, B., Gecici, O., Firidin, B., 2002d. Serum leptin and cholesterol values in the suicide attempters. Neuropsychobiology 45, 124-127.
27. Robert, D., Hare, R.D., 2004. Psychopathy Checklist-Revised (PCL-R). In: Second Edition, Technical Manual, Toronto, pp. 30-1.
28. Tang, B.L., 2008. Leptin as a neuroprotective agent. Biochem Biophys Res Commun. 368, 181-185.
29. Harvey, J., 2007. Leptin regulation of neuronal excitability and cognitive function. Curr Opin Pharmacol. 7, 643-647.
30. Harvey, J., Shanley, L.J., O’Malley, D., Irving, A.J., 2005. Leptin: a potential cognitive enhancer? Biochem Soc Trans. 33, 1029-1032.
31. Pinto, S., Roseberry, A.G., Liu, H., Diano, S., Shanabrough, M., Cai, X., Friedman, J.M., Horvath, T.L., 2004. Rapid rewiring of arcuate nucleus feeding circuits by leptin. Science. 304, 110-115.
32. Gecici, O., Kuloglu, M., Atmaca, M., Tezcan, A.E., Tunckol, H., Emül, H.M., Ustundag, B., 2005. High serum leptin levels in depressive disorders with atypical features. Psychiatry and Clinical Neurosciences. 59, 7 36-738.
33. Brown, G.L., Ebert, M.H., Goyer, P.F., Jimerson, D.C., Klein, W.J., Bunney, W.E., Goodwin, F.K., 1982. Aggression, suicide, and serotonin: Relationshipsto CSF amine metabolites. Am J Psychiatry. 139, 741-746.
34. Roy, A., Linnoila, M., 1988. Suicidal behavior, impulsivenessand serotonin. Acta Psychiatr Scand. 78, 529-535.
35. Calapai, G., Corica, F., Corsonello, A., Sautebin, L., Di Rosa, M., Campo, G.M., Buemi, M., Mauro, V.N., Caputi, A.P., 1999. Leptin increases serotoninturnover by inhibition of brain nitric oxide synthesis. J Clin Invest. 104, 975-982.
36. Matochik, J.A., London, E.D., Yildiz, B.O., Ozata, M., Caglayan, S., DePaoli, A.M., Wong, M.L., Licino, J., 2005. Effect of leptin replacement on brain structure in genetically leptin-deficient adults. J Clin Endocrinol Metab. 90, 2851-2854.
37. Baicy, K., London, E.D., Monterosso, J., Wong, M.L., Delibasi, T., Sharma, A., Licino, J., 2007. Leptin replacement alters brain response to food cues in genetically leptin-deficient adults. Proc Natl Acad Sci. 104, 18276-18279.
38. Licinio, J., Negrao, A.B., Mantzoros, C., Kaklamani, V., Wong, M.L., Bongiorno, P.B., Negro, P.P., Mulla, A., Veldhuis, J.D., Cearnal, L., Flier, J.S., Gold, P.W., 1998. Sex differences in circulating human leptin pulse amplitude: clinical implications. J. Clin. Endocrinol. Metab. 83, 4140-4147.

How to Cite this Article
Pubmed Style

Purnaningsih DP, Djawad K, Wahab S, Massi N, Alam G, Bahar B, . Protective Effect of Mangosteen Skin Extract on Albino Mice Skin with Dimethyl-Benz(a)anthracene (DMBA) Induction: Analysis on p53 Protein Level. Int J Med Rev Case Rep. 2019; 3(5): 213-215. doi:10.5455/IJMRCR.mangosteen-skin-extract-dmba


Web Style

Purnaningsih DP, Djawad K, Wahab S, Massi N, Alam G, Bahar B, . Protective Effect of Mangosteen Skin Extract on Albino Mice Skin with Dimethyl-Benz(a)anthracene (DMBA) Induction: Analysis on p53 Protein Level. http://www.mdpub.net/?mno=20988 [Access: January 29, 2020]. doi:10.5455/IJMRCR.mangosteen-skin-extract-dmba


AMA (American Medical Association) Style

Purnaningsih DP, Djawad K, Wahab S, Massi N, Alam G, Bahar B, . Protective Effect of Mangosteen Skin Extract on Albino Mice Skin with Dimethyl-Benz(a)anthracene (DMBA) Induction: Analysis on p53 Protein Level. Int J Med Rev Case Rep. 2019; 3(5): 213-215. doi:10.5455/IJMRCR.mangosteen-skin-extract-dmba



Vancouver/ICMJE Style

Purnaningsih DP, Djawad K, Wahab S, Massi N, Alam G, Bahar B, . Protective Effect of Mangosteen Skin Extract on Albino Mice Skin with Dimethyl-Benz(a)anthracene (DMBA) Induction: Analysis on p53 Protein Level. Int J Med Rev Case Rep. (2019), [cited January 29, 2020]; 3(5): 213-215. doi:10.5455/IJMRCR.mangosteen-skin-extract-dmba



Harvard Style

Purnaningsih, D. P., Djawad, . K., Wahab, . S., Massi, . N., Alam, . G., Bahar, . B. & (2019) Protective Effect of Mangosteen Skin Extract on Albino Mice Skin with Dimethyl-Benz(a)anthracene (DMBA) Induction: Analysis on p53 Protein Level. Int J Med Rev Case Rep, 3 (5), 213-215. doi:10.5455/IJMRCR.mangosteen-skin-extract-dmba



Turabian Style

Purnaningsih, Dyah Pratiwi, Khairuddin Djawad, Siswanto Wahab, Nasrum Massi, Gemini Alam, Burhanuddin Bahar, and . 2019. Protective Effect of Mangosteen Skin Extract on Albino Mice Skin with Dimethyl-Benz(a)anthracene (DMBA) Induction: Analysis on p53 Protein Level. International Journal of Medical Reviews and Case Reports, 3 (5), 213-215. doi:10.5455/IJMRCR.mangosteen-skin-extract-dmba



Chicago Style

Purnaningsih, Dyah Pratiwi, Khairuddin Djawad, Siswanto Wahab, Nasrum Massi, Gemini Alam, Burhanuddin Bahar, and . "Protective Effect of Mangosteen Skin Extract on Albino Mice Skin with Dimethyl-Benz(a)anthracene (DMBA) Induction: Analysis on p53 Protein Level." International Journal of Medical Reviews and Case Reports 3 (2019), 213-215. doi:10.5455/IJMRCR.mangosteen-skin-extract-dmba



MLA (The Modern Language Association) Style

Purnaningsih, Dyah Pratiwi, Khairuddin Djawad, Siswanto Wahab, Nasrum Massi, Gemini Alam, Burhanuddin Bahar, and . "Protective Effect of Mangosteen Skin Extract on Albino Mice Skin with Dimethyl-Benz(a)anthracene (DMBA) Induction: Analysis on p53 Protein Level." International Journal of Medical Reviews and Case Reports 3.5 (2019), 213-215. Print. doi:10.5455/IJMRCR.mangosteen-skin-extract-dmba



APA (American Psychological Association) Style

Purnaningsih, D. P., Djawad, . K., Wahab, . S., Massi, . N., Alam, . G., Bahar, . B. & (2019) Protective Effect of Mangosteen Skin Extract on Albino Mice Skin with Dimethyl-Benz(a)anthracene (DMBA) Induction: Analysis on p53 Protein Level. International Journal of Medical Reviews and Case Reports, 3 (5), 213-215. doi:10.5455/IJMRCR.mangosteen-skin-extract-dmba